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| plump granny , judystern, pills, plump nude women , depression, epa, fatty acid ester , fat asian girls , jeff bland, hardcore galleries plump rumps , plump lesbians , hammersmith hospital, plump pics , judith s. stern, vegan, | The four mutated FAS proteins together with two pairs of double mutants (OHAc/OHMa1 and SHc/SHp) were episomally expressed in appropriate delta fas1 plump black or delta fas2 deletion strains. The purified enzymes isolated plump black from these transformants were used for comparative acyl binding studies with the substrates [1- 14C]acetyl-CoA and [2-14C]malonyl-CoA. Malonate was found to be transacylated to enzyme-bound plump black pantetheine (SHc) exclusively by the Ser5421 hydroxyl group of malonyltransferase (OHMa1) while acetate could use both the acetyl (Ser819) and the malonyl (Ser5421) transferase active sites on its way to the SHc and SHp binding sites. Acylation of SHc with either substrate was unaffected by the absence of the 'peripheral' SH group (SHp) while binding of acetate to SHp was dependent on enzyme-bound pantetheine (SHc). |
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| Articles by Schweizer, E. European Journal of Biochemistry, Vol 228, 417-424, Copyright © 1995 by Federation of European Biochemical Societies ARTICLES hammersmith hospital Substrate and product binding sites of yeast fatty acid synthase. hammersmith hospital Stoichiometry and binding kinetics of wild-type and in vitro mutated enzymes H Schuster, B Rautenstrauss, M Mittag, D Stratmann and E Schweizer Lehrstuhl fur Biochemie, Universitat Erlangen-Nurnberg, Germany. The four known substrate binding sites of yeast fatty acid synthase (FAS), Ser819 (acetyltransferase, OHAC) and Ser5421 (malonyl/palmitoyl transferase, OHMa1) of subunit beta and Ser180 (pantetheine binding site, SHc) and Cys1305 (3-oxoacyl synthase, SHp) of hammersmith hospital subunit alpha were replaced, by targeted in vitro mutagenesis, by the non-acylatable amino acids glutamine, glycine or alanine. |
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