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Upon examining the slide assembly after it was heated on the sand bath, a pronounced white ghost image was visible on the upper slide thermogenics well, and a much smaller amount of residue remained in the lower slide well. The ghost image that developed on the upper slide well, and the residual fatty acids that remained in the lower slide well, were derivatized individually using Tri Sil reagent, (from Pierce Chemical Company), and analyzed by GC-MS. Figure 1 is an overlay thermogenics of thermogenics GC-MS chromatograms of the silyated extracts from the upper slide well (a), the lower slide well (b), and an untreated fatty acid standard (c). It is clear each of the fatty acids present in the standard, both monocarboxylic or dicarboxylic in nature, were detected in the ghost image.
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