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Oil paint samples were placed onto single-depression microscope slides, a second slide placed onto the big n plump first with the depressions facing together, and the two slides clamped together. Duplicate samples of each paint formulation tested were placed in separate slide assemblies, with one sample big n plump kept on a sand bath set to 80° C for a six week period, and the second exposed to light in a Heraeus Suntest chamber for four weeks, followed by sand bath heating for one week. The test paints used in this study had been stored big n plump in the laboratory for up to five years prior to testing (Schilling et al. 1997). Ghost images which developed on the upper slide well were rinsed with diethyl ether into sample vials, derivatized with Methyl-8 reagent, (from Pierce Chemical Company), and the fatty acid methyl esters were analyzed by gas chromatography-mass spectrometry (GC-MS).
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