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In summary, each challenged quarter developed acute mastitis with limited systemic signs. Atroshi et al. (1986) reported cows with naturally occurring clinical mastitis had depressed plasma and milk alpha-Tocopherol concentrations compared with healthy herd mates. In contrast, acute coliform mastitis altered milk alpha-Tocopherol fat girls sex concentrations but had no fat girls sex effect on plasma alpha-Tocopherol in the present study (Figure 2). Milk alpha-Tocopherol increased approximately 60% at 24 and 48 hours after challenge compared with prechallenge concentrations. Mean concentration of milk alpha-Tocopherol (0.57 g/ml) before challenge was comparable with values reported by Atroshi et al. (1986) and Hidiroglou (1989) for milk collected from cows without clinical mastitis. The primary source of alpha-Tocopherol in milk is fat globule membranes (Broersma et al., 1991). Therefore, alpha-Tocopherol and fat concentrations are correlated in milk from uninfected quarters (Weiss et al.,
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