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The center colony-forming units per milliliter and SCC were determined in quarter foremilk samples during the postchallenge period. Colony-forming units were determined by appropriate 10-fold dilutions of sample. The initial inocula were duplicate 1 center ml pour plates of undiluted milk in McConkey agar. Dilutions were plated on the surface of McConkey agar plates. All center dilutions were in duplicate. The SCC per milliliter of milk were determined by Fossomatic milk cell counter (Type 15600; Foss Electric, Hillerød, Denmark). Samples from clinical quarters were diluted 1:10 and 1:50 (milk:saline) for counting. Data were expressed as log10 colony-forming units per milli-liter of milk and log10 SCC per milliliter of milk. Clinical status of all quarters was recorded at the time quarter foremilk samples were obtained. Clinical status was recorded on a five-point scale: 1 = normal milk and normal quarter, 2 = normal quarter but milk was questionable, 3 = normal quarter but abnormal milk, 4 = a swollen quarter and abnormal milk, and 5 = swollen quarter, abnormal milk, and systemic signs of infection.
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