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ammonium acetate, pH 8.6) and phosphatidylcholine (PC) (5:4:1 t-BME/MeOH/0.001M ammonium acetate, pH 8.6). The purity of lipid fractions was verified by silica plump ladies HPTLC. Fatty acid methyl esters (FAME) were formed by alkaline hydrolysis of plump ladies the triacylglycerol and phospholipid fractions followed by treatment of the free fatty acids with methanolic HCl. plump ladies The FAME derivatives of fatty acids were separated by capillary gas chromatography (CGC) and analyzed using splitless injection at 200°C, a Supelco SP-2380 capillary column (30m x 0.25mm id.) in a Hewlett-Packard Model 5890 GC. The quantities of FAME were determined by converting integrated peak area data from the FID response using nonlinear regression slope data for increasing amounts (0.78-200
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