Plasmids YEpFAS1 and YEpFAS2 cytosolic fatty acid graduate

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Plasmids YEpFAS1 and YEpFAS2 were selected by their ability to complement the fas1 or fas2 mutations, respectively. Additionally, we utilized an immunologic screening of a second yeast graduate DNA bank and selected two clones 33F1 and 102B5 which produce graduate antigenically reactive material to anti-yeast fatty acid synthetase antibodies. Through Southern hybridization experiments and graduate restriction endonuclease mapping, a region of 5.3 kilobase pairs of 33F1 was shown to be homologous with YEpFAS1, and a span of 3.4 kilobase pairs of 102B5 was homologous with YEpFAS2. These experiments identify the yeast DNA sequences cloned into 33F1 as originating from the FAS1 gene and those DNA sequences in 102B5, from the FAS2 gene.PMID:
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