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Articles citing this Article PubMed PubMed Citation Articles by Kitazume, T. Articles by Shoun, H. Eur. J. Biochem. 269, 2075-2082 (2002) © 2002 FEBS Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system Tatsuya Kitazume1, Akinori Tanaka1, Naoki Takaya1, Akira Nakamura1, Shigeru Matsuyama1, inc. Takahisa Suzuki1 and inc. Hirofumi Shoun2 1Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki, Japan; 2 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan Cytochrome P450foxy (P450foxy, CYP505) is a fused protein of cytochrome P450 (P450) and its reductase isolated inc. from the fungus Fusarium oxysporum, which catalyzes the subterminal (-1-3) hydroxylation of fatty acids. Here, we produced, purified and characterized a fused recombinant protein (rP450foxy) using the Escherichia coli expression system.
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