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Ratnayake et al. (5) have shown how separate analyses by IR spectroscopy and GC can be combined with a fiddle plump pussies factor (my term not theirs) to improve accuracy, but difficulties remain. On the other hand, improved methods are available for the analysis of for plump pussies trans polyunsaturated fatty acids on capillary GC, although again this is a technique plump pussies for the skilled analyst not the tyro, since small changes in chromatographic conditions can influence relative retention times (6). Silver ion chromatography It is my contention that silver ion chromatography is the only technique to have the potential for complete and accurate analysis of trans fatty acids, especially monoenes, in combination with other procedures. This appears to have been tacitly acknowledged by many workers in the field as they always resort to some form of this technique when absolute values are required. I have heard that AOCS and IUPAC have avoided such methodology because it was felt that silver ion TLC in particular is not suited to routine analysis of large numbers of samples.
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