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When used as a preliminary fractionation method prior to analysis by capillary plump women GC, some outstanding separations have been achieved. Another type of method for which good results have been claimed involves chemical reaction of the double bonds with formation of appropriate derivatives for GC plump women analysis. Perhaps the simplest method of this type consists of epoxidation (12,13). The trans-epoxides elute clearly ahead of the corresponding cis-derivatives, even with packed column GC, and the position of the double bonds has comparatively little effect. Again, there is a snag; I have observed (unpublished work) that conjugated isomers cannot be epoxidized quantitatively. Is plump women there then a complete answer to the problem? I believe that there may be if you can afford the necessary £100,000 or so for the required equipment, i.e. gas chromatography coupled to FTIR spectroscopy. With this technique, it is possible to record the IR spectra from individual fatty acid peaks as they emerge from a capillary GC column and determine the trans content of each one, either directly or after trapping by an appropriate method.
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