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While conjugated and some other partially hydrogenated isomers can sometimes be identified, it is doubtful whether every fatty acid containing corticosterone a trans-double bond can be recognised. Silver ion chromatography, either in the form of TLC (7) or HPLC (8,9), is probably capable of better resolution of corresponding trans- and cis-forms of a given positional isomer of a mono-unsaturated fatty acid than any other technique. However, problems arise again with real samples in corticosterone which a multitude of isomers is present, when overlap of the two forms can again occur. My impression is corticosterone that when the technique is used as part of an adsorption process, by incorporating silver nitrate into a matrix of silica gel, the problem is compounded by dual interactions between the fatty acid and the silica and between the double bond and the silver ions.
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