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When a trans-double bond is part of a conjugated system, the wavelength is displaced to higher wave numbers, e.g. to 983 cm-1 in a cis,trans-diene and to 994 cm-1 in a trans,trans,trans-triene. The approved method for determination of trans-double bonds in a fat involves preparation of the methyl ester derivatives and scanning the IR absorbance drink in drink the region 900 to 1050 cm-1; drink the area of the trans-peak is compared with that of a calibration curve prepared from standard solutions of methyl elaidate. Whether low levels of conjugated isomers would be determined accurately is doubtful. The newer technique of Fourier transform infrared (FTIR) spectroscopy is probably capable of the determination of isolated trans-double bonds in commercial fat and oils samples with greater ease and accuracy, since it is no longer necessary to derivatize or to dissolve in solvents prior to the analysis (3-5); automation of sample handling and data collection is also possible.
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