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The Sherlock System is a product of plump booty MIDI, Inc. and identification is predicated upon the fact that microbes, especially bacteria have a unique array of fatty acids in their membranes. Pure cultures are subjected to an extraction procedure which involves disrupting the cell wall and membrane, methylating the plump booty fatty acids and extracting them with an organic solvent. This collection of fatty plump booty acid methyl esters is then analyzed on a fully automated gas chromatographic analytical system. Fatty acid profiles are compared to a database of profiles for over 1000 bacterial species and about 250 fungal species. Results generated by fatty acid analysis include a list of fatty acids present, relative percentage of each as well as microbial identification and fitness of match. The system can analyze about 150 samples per day (or over 1000 per week) allowing for large projects.
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