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The organic acid derivatives are separated on a capillary GC column and are identified by their mass spectral fragmentation patterns. Diseases are recognized by the identification of characteristic metabolites in sample preparation elevated concentration; and sometimes by the absence of sample preparation certain normal metabolites. C. Specimen types In this laboratory, organic acid analysis is performed routinely on urine. Cerebrospinal fluid (CSF) or vitreous humor samples can be analyzed under certain circumstances if necessary, by prior arrangement with the laboratory. 1. sample preparation Preferred Specimen Type: URINE a. Amount: optimum:2-5ml; minimum 0.5 ml) b. Collection: Any spot or timed urine may be collected, but the most diagnostically useful results are obtained from patients when they are metabolically decompensated.
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