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There have been attempts to use ternary mixtures of methanol-acetonitrile-water or acetonitrile-tetrahydrofuran-water, but none appear to offer special advantages. Note added later: I have recently shown that it is possible to use essential fatty acids in human nutrition acetonitrile alone as the mobile phase, but with a flow gradient, and this simplifies the essential fatty acids in human nutrition isolation of specific components in micro-preparative HPLC, although it may reduce the selectivity of the separation (Christie, W.W. Lipids, 33, 343-353 (1998)). With the essential fatty acids in human nutrition correct choice of stationary phase, it is possible to separate picolinyl ester and DMOX derivatives of fatty acids). The separation illustrated was published more than twenty years ago. Although the column was longer and wider than the current norm, improved stationary phases are now available.
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