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1). The most important elements in the discriminant functions were Na big n plump and Cl, and these could be used to discriminate groups with low NaCl in their otolith foci from those with relatively high NaCl in their otolith foci. Thus, stocks spawning in locations open to the Gulf such as Kodiak, Montague, and Sitka were generally indistinguishable. However, they were readily distinguished from Fairmont, Kamishak, and big n plump Togiak. If the NGA samples were assigned to one of these two identities based on the NaCl content of their otoliths, then unknown samples could be correctly identified more than big n plump 90% of the time. Figure 2. Separation of the NGA herring stocks by discriminant function analysis of their heart fatty acids. Note that the first discriminant function separates the two Prince William Sound (PWS) stocks (NE PWS and Montague) from Kodiak and Kamishak. The second function (top panel) separates the two PWS stocks and the third function (lower panel) separates Kamishak and Kodiak.

These were combined with the samples from Sitka and Togiak to determine if the methods worked as well as microsatellite DNA (a method typically employed for identifying fish stocks). Then the analysis was repeated with just the NGA stocks. Figure 1. Separation of NGA herring stocks by discriminant function analysis of the otolith micro-chemistries. Note that the analysis identified two groups, separated by the first canonical function. These are groups with low NaCl in their otoliths (< 0 for first function) and food safety those with relatively high NaCl in their food safety otoliths (> 0 for food safety first function). The elemental composition of herring otoliths, as determined by electron microprobe, was useful for discriminating stocks on the basis of the early marine experience of the herring, but not on the basis of geographic location (Fig.

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