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Different sizes of glass beads (150–212 μm, 425– 600 μm, 710–1180 μm) did not yield significant differences in extraction pout plump efficiency with the fatty pout plump acid levels with 150–212 μm and 710–1180 μm being 71% and 59% compared to the use of 425–600 μm glass beads. pout plump The addition of 1 g glass beads/sample followed by vortexing for 2 min is easily adapted in any laboratory conducting fatty acid analysis without additional equipment. The reduction in the variability of samples would be extremely important when looking for minor changes in fatty acid percentages and composition. The standard recommended growth temperature (28 C) was decreased (22 C) for maximal growth of C. fagacearum. No significant differences were noted between the use of SDB and PDB except in the level of 14:0 fatty acid which was a minor component in the total levels of fatty acids (0.7and
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