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If measurement uncertainty shall be expressed according to the ISO or EURACHEM/CITAC "Guide Quantifying Uncertainty in Analytical Measurement", the internal standard method must be adopted. The official methods of the Association of Analytical Chemists and the American Oil Chemists’s Society provide clear guidelines for subject area: ageing and mental health accurate quantification of long-chain fatty acids. Both sources stipulate the use of C23:0 (methyl tricosanoate) as internal standard and wax-type subject area: ageing and mental health capillary columns are mandatory. To help the calculation of data from peak areas we propose the use subject area: ageing and mental health of an Excel sheet to automatically convert areas given on the chromatogram (after correction if necessary) into molar percentages. Download the Excel sheet Fatty acids (column B) are ranked according to their elution order on a carbowax capillary column (column A).
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