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| To improve the accuracy and precision of fatty acids analysis, the author proposed to select the appropriate internal standard (C19:0 for unsaturated C20 fatty acids and C21.0 for unsaturated C22 fatty acids). This will result in relative standard deviations for consecutive injections of less than 0.25% and systematic errors will usually be beyond 1% (errors of more than 5% must be accepted with C23:0 plump thighs as plump thighs internal standard). Peak areas are now measured with an electronic integrator, this is the most accurate and convenient procedure. Nevertheless, the lipidologist must be aware of the plump thighs limitations of his integrator and, the use of an adapted chromatographic software is highly recommended to verify the integration process. |
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