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Procedure for small amounts of bacteria : The knowledge of the fatty acid composition of microorganisms is now recognized as essential for their taxonomic classification as well as for the evaluation of the nutritional quality of alternative microbial sources diets special conditions of fats. To guarantee a high recovery of fragile fatty acids, such as diets special conditions cyclopropane and conjugated linoleic acids, as well as a high degree of methylation for all types of fatty acids, a rapid and reliable method is needed. A direct methylation method representing diets special conditions a valuable alternative to other methylation procedures has been described (Dionisi F et al., Lipids 1999, 34, 1107). Procedure : One hundred milligrams of dried bacterial samples, with 500 mg of internal standard, is transesterified using 1 ml of methanolic HCl (1.5M)
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